@article {1333945,
	title = {Molecular layer deposition builds biocompatible substrates for epithelial cells},
	journal = {J Biomed Mater Res A},
	volume = {106},
	number = {12},
	year = {2018},
	month = {2018 Dec},
	pages = {3090-3098},
	abstract = {The demand for novel biocompatible materials as surface coating in the field of regenerative medicine is high. We explored molecular layer deposition (MLD) technique for building surface coatings and introduced a new group of substrates consisting of amino acids, or nucleobases, and the biocompatible metal titanium. The substrates were built from titanium tetraisopropoxide (TTIP) with l-lysine, glycine, l-aspartic acid, l-arginine, thymine, uracil, and adenine. Substrates based on zirconium chloride and terephthalic acid were also included. Titanium oxide (TiO ) substrates made by atomic layer deposition and uncoated cover slips served as controls. Rat conjunctival epithelial goblet cells were grown in RPMI 1640 and RT-PCR, immunofluorescence, cell attachment, proliferation, and viability were analyzed. Cells cultured on MLD and uncoated substrates were proliferating (positive for Ki67). Cell attachment after 3 h of culture on MLD substrates was similar to uncoated coverslips (p \&gt; 0.05). Compared to uncoated coverslips, cell proliferation assayed with alamarBlue{\textregistered} after 4 days was significantly higher on all MLD substrates (p \&lt; 0.05), whereas terephthalic acid-containing MLD substrates reduced proliferation (p \&lt; 0.01). Viability assessed by LIVE/DEAD{\textregistered} was high (\&gt;85\%) for all substrates after 5 days. The novel MLD technique is promising for building biocompatible substrates that direct epithelial cell growth. {\textcopyright} 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3090-3098, 2018.},
	issn = {1552-4965},
	doi = {10.1002/jbm.a.36499},
	author = {Momtazi, Leva and Dartt, Darlene A. and Nilsen, Ola and Eidet, Jon Roger}
}
