@article {1351155,
	title = {Transscleral delivery of Nd: YLF laser at 1,047 nm causes vascular occlusion in experimental pigmented choroidal melanoma},
	journal = {Retina},
	volume = {34},
	number = {4},
	year = {2014},
	month = {2014 Apr},
	pages = {792-800},
	abstract = {PURPOSE: The aims of this study were to determine the scleral attenuation of focused neodymium: yttrium-lanthanum-fluoride laser at 1,047 nm applied transsclerally and whether transscleral delivery can close the vascular supply at the base of experimental choroidal melanoma in rabbits. METHODS: Fifty-two New Zealand albino rabbits were included. Scleral laser attenuation was measured across fresh sclera. B16F10 melanomas were established in the subchoroidal space of 49 rabbits. Twenty-one animals were killed immediately after transscleral treatment, 14 were followed for 2 weeks to 4 weeks, and 14 were followed without treatment. Ophthalmoscopy, fundus photographs, and fluorescein angiography were performed before treatment, immediately after, and weekly during the follow-up. Eyes were examined by light microscopy. RESULTS: Sclera attenuated laser energy by 31\% {\textpm} 7\%. Immediately after treatment, angiography showed diffuse hypofluorescence in 71\% (15 of 21 rabbits). Light microscopy showed vascular occlusion extending at least two thirds of the tumor thickness from the base. Seven of the 14 tumors followed for 15 days {\textpm} 8 days were eradicated. There was no correlation between tumor height and eradication. CONCLUSION: Rabbit sclera attenuated 31\% {\textpm} 7\% of laser energy. A single transscleral treatment causes tumor vascular closure at the base and may serve as an adjuvant therapy to ensure destruction of deep and intrascleral tumor cells.},
	keywords = {Animals, Choroid Neoplasms, Female, Fluorescein Angiography, Laser Coagulation, Lasers, Solid-State, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Neovascularization, Pathologic, Ophthalmoscopy, Rabbits, Sclera, Tumor Cells, Cultured},
	issn = {1539-2864},
	doi = {10.1097/IAE.0b013e3182a2e723},
	author = {Damico, Francisco Max and Takahashi, Beatriz S and Acquesta, Felipe B}
}
