@article {1351218,
	title = {Promoter decommissioning by the NuRD chromatin remodeling complex triggers synaptic connectivity in the mammalian brain},
	journal = {Neuron},
	volume = {83},
	number = {1},
	year = {2014},
	month = {2014 Jul 02},
	pages = {122-34},
	abstract = {Precise control of gene expression plays fundamental roles in brain development, but the roles of chromatin regulators in neuronal connectivity have remained poorly understood. We report that depletion of the NuRD complex by in\&nbsp;vivo RNAi and conditional knockout of the core NuRD subunit Chd4 profoundly impairs the establishment of granule neuron parallel fiber/Purkinje cell synapses in the rodent cerebellar cortex in\&nbsp;vivo. By interfacing genome-wide sequencing of transcripts and ChIP-seq analyses, we uncover a network of repressed genes and distinct histone modifications at target gene promoters that are developmentally regulated by the NuRD complex in the cerebellum in\&nbsp;vivo. Finally, in a targeted in\&nbsp;vivo RNAi screen of NuRD target genes, we identify a program of NuRD-repressed genes that operate as critical regulators of presynaptic differentiation in the cerebellar cortex. Our findings define NuRD-dependent promoter decommissioning as a developmentally regulated programming mechanism that drives synaptic connectivity in the mammalian brain.},
	keywords = {Animals, Animals, Newborn, Brain Chemistry, Cells, Cultured, Chromatin Assembly and Disassembly, DNA-Binding Proteins, Humans, Mi-2 Nucleosome Remodeling and Deacetylase Complex, Mice, Mice, Knockout, Mice, Transgenic, Promoter Regions, Genetic, Purkinje Cells, Rats, Rats, Long-Evans, Rats, Sprague-Dawley, Retinoblastoma-Binding Protein 4, Synapses},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2014.05.039},
	author = {Yamada, Tomoko and Yang, Yue and Hemberg, Martin and Yoshida, Toshimi and Cho, Ha Young and Murphy, J Patrick and Fioravante, Diasynou and Regehr, Wade G and Gygi, Steven P and Georgopoulos, Katia and Bonni, Azad}
}
