@article {1364530,
	title = {Heat shock protein 27 mediated signaling in viral infection},
	journal = {Biochemistry},
	volume = {51},
	number = {28},
	year = {2012},
	month = {2012 Jul 17},
	pages = {5695-702},
	abstract = {Heat shock proteins (HSPs) play a critical role in many intracellular processes, including apoptosis and delivery of other proteins to intracellular compartments. Small HSPs have been shown previously to participate in many cellular functions, including IL-8 induction. Human adenovirus infection activates intracellular signaling, involving particularly the c-Src and mitogen-activated protein kinases [Natarajan, K., et al. (2003) J. Immunol. 170, 6234-6243]. HSP27 and MK2 are also phosphorylated, and c-Src, and its downstream targets, p38, ERK1/2, and c-Jun-terminal kinase (JNK), differentially mediate IL-8 and MCP-1 expression. Specifically, activation and translocation of transcription factor NFκB-p65 occurs in a p38-dependent fashion [Rajaiya, J., et al. (2009) Mol. Vision 15, 2879-2889]. Herein, we report a novel role for HSP27 in an association of p38 with NFκB-p65. Immunoprecipitation assays of virus-infected but not mock-infected cells revealed a signaling complex including p38 and NFκB-p65. Transfection with HSP27 short interfering RNA (siRNA) but not scrambled RNA disrupted this association and reduced the level of IL-8 expression. Transfection with HSP27 siRNA also reduced the level of nuclear localization of NFκB-p65 and p38. By use of tagged p38 mutants, we found that amino acids 279-347 of p38 are necessary for the association of p38 with NFκB-p65. These studies strongly suggest that HSP27, p38, and NFκB-p65 form a signalosome in virus-infected cells and influence downstream expression of pro-inflammatory mediators.},
	keywords = {Adenovirus Infections, Human, Adenoviruses, Human, Cells, Cultured, Chemokines, HSP27 Heat-Shock Proteins, Humans, Keratinocytes, NF-kappa B p50 Subunit, p38 Mitogen-Activated Protein Kinases, Protein Transport, Signal Transduction},
	issn = {1520-4995},
	doi = {10.1021/bi3007127},
	author = {Rajaiya, Jaya and Yousuf, Mohammad A and Singh, Gurdeep and Stanish, Heather and Chodosh, James}
}
