@article {1364571,
	title = {Characterization of the interaction between hydroxypropyl guar galactomannan and galectin-3},
	journal = {Biochem Biophys Res Commun},
	volume = {424},
	number = {1},
	year = {2012},
	month = {2012 Jul 20},
	pages = {12-7},
	abstract = {Multivalent galactose ligands have been proposed for selective targeting of carbohydrate-binding proteins on epithelial cell surfaces, both in normal and pathological conditions. One cellular partner is galectin-3, a β-galactoside-binding protein present on many epithelial linings, such as those of the ocular surface. In this study, we investigated the ability of hydroxypropyl guar galactomannan (HPGG) to bind recombinant galectin-3 and to target the apical surface of differentiated human corneal keratinocytes. Pull-down and slot-blot assays demonstrated that fluorescence-labeled HPGG bound recombinant galectin-3 through a galactose-dependent mechanism. In contrast, no binding of HPGG could be detected towards recombinant galectin-8 or -9. In a cell culture system, HPGG bound weakly to biotinylated cell surface corneal isolates containing endogenous galectin-3, and incubation of HPGG with corneal keratinocytes in culture resulted in discrete, galactose-independent, binding to the cell surface. Moreover, HPGG failed to elute the biological counter-receptor MUC16 from galectin-3 affinity columns. We conclude that HPGG binds galectin-3 through the conventional carbohydrate-recognition domain in vitro, but not in a biological system, suggesting that endogenous carbohydrate ligands on epithelial cell surface glycocalyces impair HPGG biorecognition.},
	keywords = {CA-125 Antigen, Cell Line, Cornea, Galactose, Galectin 3, Humans, Keratinocytes, Mannans, Membrane Proteins},
	issn = {1090-2104},
	doi = {10.1016/j.bbrc.2012.05.160},
	author = {Woodward, Ashley M and Senchyna, Michelle and Williams, Ravaughn and Arg{\"u}eso, Pablo}
}
