@article {1435401,
	title = {In Situ Modification of Tissue Stem and Progenitor Cell Genomes},
	journal = {Cell Rep},
	volume = {27},
	number = {4},
	year = {2019},
	month = {2019 Apr 23},
	pages = {1254-1264.e7},
	abstract = {In\&nbsp;vivo delivery of genome-modifying enzymes holds significant promise for therapeutic applications and functional genetic screening. Delivery to endogenous tissue stem cells, which provide an enduring source of cell replacement during homeostasis and regeneration, is of particular interest. Here, we use a sensitive Cre/lox fluorescent reporter system to test the efficiency of genome modification following in\&nbsp;vivo transduction by adeno-associated viruses (AAVs) in tissue stem and progenitor cells. We combine immunophenotypic analyses with in\&nbsp;vitro and in\&nbsp;vivo assays of stem cell function to reveal effective targeting of skeletal muscle satellite cells,\&nbsp;mesenchymal progenitors, hematopoietic stem cells, and dermal cell subsets using multiple AAV serotypes. Genome modification rates achieved through this system reached \&gt;60\%, and modified cells retained key functional properties. This study establishes a powerful platform to genetically alter tissue progenitors within their physiological niche while preserving their native stem cell properties and regulatory interactions.},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2019.03.105},
	author = {Goldstein, Jill M and Tabebordbar, Mohammadsharif and Zhu, Kexian and Wang, Leo D and Messemer, Kathleen A and Peacker, Bryan and Ashrafi Kakhki, Sara and Meryem Gonzalez-Celeiro and Shwartz, Yulia and Cheng, Jason K W and Xiao, Ru and Barungi, Trisha and Albright, Charles and Ya-Chieh Hsu and Vandenberghe, Luk H and Wagers, Amy J}
}
