@article {1470984,
	title = {Regulatory T cells promote corneal endothelial cell survival following transplantation via interleukin-10},
	journal = {Am J Transplant},
	volume = {20},
	number = {2},
	year = {2020},
	month = {2020 Feb},
	pages = {389-398},
	abstract = {The functional competence of corneal endothelial cells (CEnCs) is critical for survival of corneal allografts, but these cells are often targets of the immune response mediated by graft-attacking effector T cells. Although regulatory T cells (Tregs) have been studied for their role in regulating the host{\textquoteright}s alloimmune response towards the graft, the cytoprotective function of these cells on CEnCs has not been investigated. The aim of this study was to determine whether Tregs suppress effector T cell-mediated and inflammatory cytokine-induced CEnC death, and to elucidate the mechanism by which this cytoprotection occurs. Using 2 well-established models of corneal transplantation (low-risk and high-risk models), we show that Tregs derived from low-risk graft recipients have a superior capacity in protecting CEnCs against effector T cell-mediated and interferon-γ and tumor necrosis factor-α-induced cell death compared to Tregs derived from high-risk hosts. We further demonstrate that the cytoprotective function of Tregs derived from low-risk hosts occurs independently of direct cell-cell contact and is mediated by the immunoregulatory cytokine IL-10. Our study is the first to report that Tregs provide cytoprotection for CEnCs through secretion of IL-10, indicating potentially novel therapeutic targets for enhancing CEnC survival following corneal transplantation.},
	issn = {1600-6143},
	doi = {10.1111/ajt.15631},
	author = {Coco, Giulia and Foulsham, William and Nakao, Takeshi and Yin, Jia and Amouzegar, Afsaneh and Taketani, Yukako and Chauhan, Sunil K and Dana, Reza}
}
