@article {1478336, title = {High-throughput dense reconstruction of cell lineages}, journal = {Open Biol}, volume = {9}, number = {12}, year = {2019}, month = {2019 Dec}, pages = {190229}, abstract = {The first meeting exclusively dedicated to the {\textquoteright}High-throughput dense reconstruction of cell lineages{\textquoteright} took place at Janelia Research Campus (Howard Hughes Medical Institute) from 14 to 18 April 2019. Organized by Tzumin Lee, Connie Cepko, Jorge Garcia-Marques and Isabel Espinosa-Medina, this meeting echoed the recent eruption of new tools that allow the reconstruction of lineages based on the phylogenetic analysis of DNA mutations induced during development. Combined with single-cell RNA sequencing, these tools promise to solve the lineage of complex model organisms at single-cell resolution. Here, we compile the conference consensus on the technological and computational challenges emerging from the use of the new strategies, as well as potential solutions.}, issn = {2046-2441}, doi = {10.1098/rsob.190229}, author = {Espinosa-Medina, Isabel and Garcia-Marques, Jorge and Cepko, Connie and Lee, Tzumin} }