@article {1490444,
	title = {Resident plasmacytoid dendritic cells patrol vessels in the na{\"\i}ve limbus and conjunctiva},
	journal = {Ocul Surf},
	volume = {18},
	number = {2},
	year = {2020},
	month = {2020 Apr},
	pages = {277-285},
	abstract = {Plasmacytoid dendritic cells (pDCs) constitute a unique population of bone marrow-derived cells that play a pivotal role in linking innate and adaptive immune responses. While peripheral tissues are typically devoid of pDCs during steady state, few tissues do host resident pDCs. In the current study, we aim to assess presence and distribution of pDCs in na{\"\i}ve murine limbus and bulbar conjunctiva. Immunofluorescence staining followed by confocal microscopy revealed that the na{\"\i}ve bulbar conjunctiva of wild-type mice hosts CD45 CD11c PDCA-1 pDCs. Flow cytometry confirmed the presence of resident pDCs in the bulbar conjunctiva through multiple additional markers, and showed that they express maturation markers, the T cell co-inhibitory molecules PD-L1 and B7-H3, and minor to negligible levels of T cell co-stimulatory molecules CD40, CD86, and ICAM-1. Epi-fluorescent microscopy of DPE-GFP{\texttimes}RAG1 transgenic mice with GFP-tagged pDCs indicated lower density of pDCs in the bulbar conjunctiva compared to the limbus. Further, intravital multiphoton microscopy revealed that resident pDCs accompany the limbal vessels and patrol the intravascular space. In vitro multiphoton microscopy showed that pDCs are attracted to human umbilical vein endothelial cells and interact with them during tube formation. In conclusion, our study shows that the limbus and bulbar conjunctiva are endowed with resident pDCs during steady state, which express maturation and classic T cell co-inhibitory molecules, engulf limbal vessels, and patrol intravascular spaces.},
	issn = {1937-5913},
	doi = {10.1016/j.jtos.2020.02.005},
	author = {Jamali, Arsia and Harris, Deshea L and Blanco, Tomas and Lopez, Maria J and Hamrah, Pedram}
}
