@article {1522742, title = {Stem Cell-Derived Viral Antigen-Specific T Cells Suppress HBV Replication through Production of IFN-γ and TNF-⍺}, journal = {iScience}, volume = {23}, number = {7}, year = {2020}, month = {2020 Jul 24}, pages = {101333}, abstract = {The viral antigen (Ag)-specific CD8 cytotoxic T lymphocytes (CTLs) derived from pluripotent stem cells (PSCs), i.e., PSC-CTLs, have the ability to suppress hepatitis B virus (HBV) infection. After adoptive transfer, PSC-CTLs can infiltrate into the liver to suppress HBV replication. Nevertheless, the mechanisms by which the viral Ag-specific PSC-CTLs provoke the antiviral response remain to be fully elucidated. In this study, we generated the functional HBV surface Ag-specific CTLs from the induced PSC (iPSCs), i.e., iPSC-CTLs, and investigated the underlying mechanisms of the CTL-mediated antiviral replication in a murine model. We show that adoptive transfer of HBV surface Ag-specific iPSC-CTLs greatly suppressed HBV replication and prevented HBV surface Ag expression. We further demonstrate that the adoptive transfer significantly increased T\ cell accumulation and production of antiviral cytokines. These results indicate that stem cell-derived viral Ag-specific CTLs can robustly accumulate in the liver and suppress HBV replication through producing antiviral cytokines.}, issn = {2589-0042}, doi = {10.1016/j.isci.2020.101333}, author = {Haque, Mohammad and Lei, Fengyang and Xiong, Xiaofang and Ren, Yijie and Kumar, Anil and Das, Jugal Kishore and Ren, Xingcong and Fang, Deyu and de Figueiredo, Paul and Yang, Jin-Ming and Song, Jianxun} }