@article {1573104, title = {An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells}, journal = {STAR Protoc}, volume = {2}, number = {1}, year = {2021}, month = {2021 Mar 19}, pages = {100264}, abstract = {In T\ cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8 cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T\ cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et\ al. (2020).}, issn = {2666-1667}, doi = {10.1016/j.xpro.2020.100264}, author = {Haque, Mohammad and Xiong, Xiaofang and Lei, Fengyang and Das, Jugal Kishore and Song, Jianxun} }