@article {1608606,
	title = {An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primates},
	journal = {Cell Host Microbe},
	volume = {29},
	number = {9},
	year = {2021},
	month = {2021 09 08},
	pages = {1437-1453.e8},
	abstract = {The SARS-CoV-2 pandemic has affected more than 185 million people worldwide resulting in over 4 million deaths. To contain the pandemic, there is a continued need for safe vaccines that provide durable protection at low and scalable doses and can be deployed easily. Here, AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity in mouse and non-human primates following a single injection and confers complete protection from SARS-CoV-2 challenge in macaques. Peak neutralizing antibody titers are sustained at 1 year and complemented by functional memory T\&nbsp;cell responses. The AAVCOVID vector has no relevant pre-existing immunity in humans and does not elicit cross-reactivity to common AAVs used in gene therapy. Vector genome persistence and expression wanes following injection. The single low-dose requirement, high-yield manufacturability, and 1-month stability for storage at room temperature may make this technology well suited to support effective immunization campaigns for emerging pathogens on a global scale.},
	keywords = {Animals, Antibodies, Neutralizing, Antibodies, Viral, COVID-19, COVID-19 Vaccines, Dependovirus, Female, Humans, Immunogenicity, Vaccine, Immunologic Memory, Macaca fascicularis, Macaca mulatta, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, SARS-CoV-2, Spike Glycoprotein, Coronavirus, T-Lymphocytes, Transgenes, Vaccination, Viral Load},
	issn = {1934-6069},
	doi = {10.1016/j.chom.2021.08.002},
	author = {Zabaleta, Nerea and Dai, Wenlong and Bhatt, Urja and H{\'e}rate, C{\'e}cile and Maisonnasse, Pauline and Chichester, Jessica A and Sanmiguel, Julio and Estelien, Reynette and Michalson, Kristofer T and Diop, Cheikh and Maciorowski, Dawid and Dereuddre-Bosquet, Nathalie and Cavarelli, Mariangela and Gallou{\"e}t, Anne-Sophie and Naninck, Thibaut and Kahlaoui, Nidhal and Lemaitre, Julien and Qi, Wenbin and Hudspeth, Elissa and Cucalon, Allison and Dyer, Cecilia D and Pampena, M Betina and Knox, James J and LaRocque, Regina C and Charles, Richelle C and Dan Li and Kim, Maya and Sheridan, Abigail and Storm, Nadia and Johnson, Rebecca I and Feldman, Jared and Hauser, Blake M and Contreras, Vanessa and Marlin, Romain and Tsong Fang, Rapha{\"e}l Ho and Chapon, Catherine and van der Werf, Sylvie and Zinn, Eric and Ryan, Aisling and Kobayashi, Dione T and Chauhan, Ruchi and McGlynn, Marion and Ryan, Edward T and Schmidt, Aaron G and Price, Brian and Honko, Anna and Griffiths, Anthony and Yaghmour, Sam and Hodge, Robert and Betts, Michael R and Freeman, Mason W and Wilson, James M and Le Grand, Roger and Vandenberghe, Luk H}
}
