@article {1638554,
	title = {Sleep deprivation induces corneal epithelial progenitor cell over-expansion through disruption of redox homeostasis in the tear film},
	journal = {Stem Cell Reports},
	volume = {17},
	number = {5},
	year = {2022},
	month = {2022 May 10},
	pages = {1105-1119},
	abstract = {Sleep deficiency, a common public health problem, causes ocular discomfort and affects ocular surface health. However, the underlying mechanism remains unclear. Herein, we identified that short-term sleep deprivation (SD) resulted in hyperproliferation of corneal epithelial progenitor cells (CEPCs) in mice. The expression levels of p63 and Keratin 14, the biomarkers of CEPCs, were upregulated in the corneal epithelium after short-term SD. In addition, SD led to elevated levels of reactive oxygen species (ROS), and subsequent decrease in antioxidant capacity, in the tear film. Exogenous hydrogen peroxide (H2O2) could directly stimulate the proliferation of CEPCs in\&nbsp;vivo and in\&nbsp;vitro. Topical treatment of antioxidant L-glutathione preserved the over-proliferation of CEPCs and attenuated corneal epithelial defects in SD mice. Moreover, the activation of the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway is essential to ROS-stimulated cell proliferation in CEPCs. However, long-term SD ultimately led to early manifestation of limbal stem cell deficiency.},
	keywords = {Animals, Antioxidants, Cell Proliferation, Epithelium, Corneal, Homeostasis, Hydrogen Peroxide, Mice, Oxidation-Reduction, Phosphatidylinositol 3-Kinases, Reactive Oxygen Species, Sleep Deprivation, Stem Cells},
	issn = {2213-6711},
	doi = {10.1016/j.stemcr.2022.03.017},
	author = {Li, Sanming and Tang, Liying and Zhou, Jing and Anchouche, Sonia and Li, Dian and Yang, Yiran and Liu, Zhaolin and Wu, Jieli and Hu, Jiaoyue and Zhou, Yueping and Yin, Jia and Liu, Zuguo and Li, Wei}
}
