@article {1661812,
	title = {Transcorneal but not transpalpebral electrical stimulation disrupts mucin homeostasis of the ocular surface},
	journal = {BMC Ophthalmol},
	volume = {22},
	number = {1},
	year = {2022},
	month = {2022 Dec 15},
	pages = {490},
	abstract = {PURPOSE: Transcorneal electrical stimulation (TcES) is increasingly applied as a therapy for preserving and improving vision in retinal neurodegenerative and ischemic disorders. However, a common complaint about TcES is its induction of eye pain and dryness in the clinic, while the mechanisms remain unknown. METHOD: TcES or transpalpebral ES (TpES) was conducted in C57BL6j mice for 14 days. The contralateral eyes were used as non-stimulated controls. Levels of intracellular [Ca2+] ([Ca2+]i) were assessed by Fura-2AM. The conductance resistances of the eye under various ES conditions were measured in vivo by an oscilloscope. RESULTS: Although TcES did not affect tear production, it significantly induced damage to the ocular surface, as revealed by corneal fluorescein staining that was accompanied by significantly decreased mucin (MUC) 4 expression compared to the control. Similar effects of ES were detected in cultured primary corneal epithelium cells, showing decreased MUC4 and ZO-1 levels after the ES in vitro. In addition, TcES decreased secretion of MUC5AC from the conjunctiva in vivo, which was also corroborated in goblet cell cultures, where ES significantly attenuated carbachol-induced [Ca2+]i increase. In contrast to TcES, transpalpebral ES (TpES) did not induce corneal fluorescein staining while significantly increasing tear production. Importantly, the conductive resistance from orbital skin to the TpES was significantly smaller than that from the cornea to the retina in TcES. CONCLUSION: TcES, but not TpES, induces corneal epithelial damage in mice by disrupting mucin homeostasis. TpES thus may represent a safer and more effective ES approach for treating retinal neurodegeneration clinically.},
	keywords = {Animals, Conjunctiva, Dry Eye Syndromes, Electric Stimulation, Fluorescein, Goblet Cells, Homeostasis, Mice, Tears},
	issn = {1471-2415},
	doi = {10.1186/s12886-022-02717-z},
	author = {Yang, Menglu and Lennikov, Anton and Chang, Karen and Ashok, Ajay and Lee, Cherin and Cho, Kin-Sang and Utheim, Tor Paaske and Dartt, Darlene A. and Chen, Dong Feng}
}
