@article {314121,
	title = {Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.},
	journal = {EMBO Mol Med},
	volume = {6},
	number = {9},
	year = {2014},
	month = {2014 Sep},
	pages = {1175-90},
	abstract = {In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (~100\&nbsp;Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.},
	issn = {1757-4684},
	doi = {10.15252/emmm.201404077},
	author = {Cronin, Therese and Vandenberghe, Luk H and Hantz, P{\'e}ter and Juttner, Josephine and Reimann, Andreas and Kacs{\'o}, Agota-Enik{\H o} and Huckfeldt, Rachel M and Busskamp, Volker and Kohler, Hubertus and Lagali, Pamela S and Roska, Botond and Bennett, Jean}
}
