@article {314186,
	title = {Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids},
	journal = {Proc Natl Acad Sci U S A},
	volume = {111},
	number = {34},
	year = {2014},
	month = {2014 Aug 26},
	pages = {12510-5},
	abstract = {Staphylococcus aureus contains two distinct teichoic acid (TA) polymers, lipoteichoic acid (LTA) and wall teichoic acid (WTA), which are proposed to play redundant roles in regulating cell division. To gain insight into the underlying biology of S. aureus TAs, we used a small molecule inhibitor to screen a highly saturated transposon library for cellular factors that become essential when WTA is depleted. We constructed an interaction network connecting WTAs with genes involved in LTA synthesis, peptidoglycan synthesis, surface protein display, and D-alanine cell envelope modifications. Although LTAs and WTAs are synthetically lethal, we report that they do not have the same synthetic interactions with other cell envelope genes. For example, D-alanylation, a tailoring modification of both WTAs and LTAs, becomes essential when the former, but not the latter, are removed. Therefore, D-alanine-tailored LTAs are required for survival when WTAs are absent. Examination of terminal phenotoypes led to the unexpected discovery that cells lacking both LTAs and WTAs lose their ability to form Z rings and can no longer divide. We have concluded that the presence of either LTAs or WTAs on the cell surface is required for initiation of S. aureus cell division, but these polymers act as part of distinct cellular networks.},
	keywords = {Alanine, Cell Division, Cell Wall, Chromosome Mapping, DNA Transposable Elements, Gene Knockout Techniques, Gene Regulatory Networks, Genes, Bacterial, Lipopolysaccharides, Mutation, Phenotype, Staphylococcus aureus, Teichoic Acids},
	issn = {1091-6490},
	doi = {10.1073/pnas.1404099111},
	author = {Santa Maria, John P and Sadaka, Ama and Moussa, Samir H and Brown, Stephanie and Zhang, Yanjia J and Rubin, Eric J and Gilmore, Michael S and Walker, Suzanne}
}
