@article {509141, title = {Cell type-specific manipulation with GFP-dependent Cre recombinase.}, journal = {Nat Neurosci}, volume = {18}, number = {9}, year = {2015}, month = {2015 Sep}, pages = {1334-41}, abstract = {There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP(+) cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins.}, issn = {1546-1726}, doi = {10.1038/nn.4081}, author = {Tang, Jonathan C Y and Rudolph, Stephanie and Dhande, Onkar S and Abraira, Victoria E and Choi, Seungwon and Lapan, Sylvain W and Drew, Iain R and Drokhlyansky, Eugene and Huberman, Andrew D and Regehr, Wade G and Cepko, Constance L} }