@article {541266,
	title = {Comparison of Standard Versus Wide-Field Composite Images of the Corneal Subbasal Layer by In Vivo Confocal Microscopy.},
	journal = {Invest Ophthalmol Vis Sci},
	volume = {56},
	number = {10},
	year = {2015},
	month = {2015 Sep 1},
	pages = {5801-7},
	abstract = {PURPOSE: To evaluate whether the densities of corneal subbasal nerves and epithelial immune dendritiform cells (DCs) are comparable between a set of three representative standard images of in vivo confocal microscopy (IVCM) and the wide-field mapped composite IVCM images. METHODS: This prospective, cross-sectional, and masked study included 110 eyes of 58 patients seen in a neurology clinic who underwent laser-scanning IVCM (Heidelberg Retina Tomograph 3) of the central cornea. Densities of subbasal corneal nerves and DCs were compared between the average of three representative standard images and the wide-field mapped composite images, which were reconstructed by automated mapping. RESULTS: There were no statistically significant differences between the average of three representative standard images (0.16 mm2 each) and the wide-field composite images (1.29 {\textpm} 0.64 mm2) in terms of mean subbasal nerve density (17.10 {\textpm} 6.10 vs. 17.17 {\textpm} 5.60 mm/mm2, respectively, P = 0.87) and mean subbasal DC density (53.2 {\textpm} 67.8 vs. 49.0 {\textpm} 54.3 cells/mm2, respectively, P = 0.43). However, there were notable differences in subbasal nerve and DC densities between these two methods in eyes with very low nerve density or very high DC density. CONCLUSIONS: There are no significant differences in the mean subbasal nerve and DC densities between the average values of three representative standard IVCM images and wide-field mapped composite images. Therefore, these standard images can be used in clinical studies to accurately measure cellular structures in the subbasal layer.},
	issn = {1552-5783},
	doi = {10.1167/iovs.15-17434},
	author = {Kheirkhah, Ahmad and Muller, Rodrigo and Mikolajczak, Janine and Ren, Ai and Kadas, Ella Maria and Zimmermann, Hanna and Pruess, Harald and Paul, Friedemann and Brandt, Alexander U and Hamrah, Pedram}
}
