@article {541351,
	title = {Stamping an S on DMEK Donor Tissue to Prevent Upside-Down Grafts: Laboratory Validation and Detailed Preparation Technique Description.},
	journal = {Cornea},
	volume = {34},
	number = {9},
	year = {2015},
	month = {2015 Sep},
	pages = {1175-8},
	abstract = {PURPOSE: To report endothelial cell loss (ECL) caused by a novel S-stamp preparation technique for Descemet membrane endothelial keratoplasty (DMEK). METHODS: Six cadaveric human corneas were prepared for DMEK transplantation using a single standardized technique, including the application of a dry ink gentian violet S-stamp to the stromal side of Descemet membrane. Endothelial cell death was evaluated and quantified using computerized analysis of vital dye staining. RESULTS: ECL caused by the S-stamp was 0.6\% (range 0.1\%-1.0\%), which comprised less than one-tenth of the total ECL caused by our preparation of the DMEK graft from the start to finish, including recovery, prestripping, S-stamping, and trephination (13.7\% total ECL, range 9.9\%-17.6\%). CONCLUSIONS: Our novel S-stamp donor tissue preparation technique is intuitive to learn and holds the promise of preventing iatrogenic primary graft failure due to upside-down grafts without causing unacceptable increases in ECL.},
	issn = {1536-4798},
	doi = {10.1097/ICO.0000000000000522},
	author = {Veldman, Peter B and Dye, Philip K and Holiman, Jeffrey D and Mayko, Zachary M and S{\'a}les, Christopher S and Straiko, Michael D and Stoeger, Christopher G and Terry, Mark A}
}
