@article {647361, title = {Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions.}, journal = {Nat Commun}, volume = {7}, year = {2016}, month = {2016}, pages = {10363}, abstract = {Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, a primary site of leukocyte recruitment during inflammation. Here we show that endomucin abrogation on quiescent endothelial cells enables neutrophils to adhere firmly, via LFA-1-mediated binding to ICAM-1 constitutively expressed by endothelial cells. Moreover, TNF-α stimulation downregulates cell surface expression of endomucin concurrent with increased expression of adhesion molecules. Adenovirus-mediated expression of endomucin under inflammatory conditions prevents neutrophil adhesion in vitro and reduces the infiltration of CD45(+) and NIMP-R14(+) cells in vivo. These results indicate that endomucin prevents leukocyte contact with adhesion molecules in non-inflamed tissues and that downregulation of endomucin is critical to facilitate adhesion of leukocytes into inflamed tissues.}, issn = {2041-1723}, doi = {10.1038/ncomms10363}, author = {Zahr, Alisar and Alcaide, Pilar and Yang, Jinling and Jones, Alexander and Gregory, Meredith and Dela Paz, Nathaniel G and Patel-Hett, Sunita and Nevers, Tania and Koirala, Adarsha and Luscinskas, Francis W and Saint-Geniez, Magali and Ksander, Bruce and D{\textquoteright}Amore, Patricia A and Arg{\"u}eso, Pablo} }