@article {655056,
	title = {E-Selectin Mediates Immune Cell Trafficking in Corneal Transplantation.},
	journal = {Transplantation},
	volume = {100},
	number = {4},
	year = {2016},
	month = {2016 Apr},
	pages = {772-80},
	abstract = {BACKGROUND: Immune rejection continues to threaten all tissue transplants. Here we sought to determine whether platelet (P)- and endothelial (E)-selectin mediate T cell recruitment in corneal transplantation and whether their blockade can reduce T cell graft infiltration and improve long-term corneal allograft survival. METHODS: In a murine model of allogeneic corneal transplantation, we used PCR and immunohistochemistry to investigate expression of P- and E-selectin in rejected versus accepted allografts and lymph node flow cytometry to assess expression of selectin ligands by effector T cells. Using P- and E-selectin neutralizing antibodies, we evaluated the effect of blockade on CD4 T cell recruitment, as well as the effect of anti-E-selectin on long-term allograft survival. RESULTS: The P- (93.3-fold, P \&lt; 0.05) and E-selectin (17.1-fold, P \&lt; 0.005) are upregulated in rejected versus accepted allogeneic transplants. Type 1 T helper cells from hosts with accepted and rejected grafts express high levels of P-selectin glycoprotein ligand 1 and glycosylated CD43. In vivo blockade of P (0.47 {\textpm} 0.03, P \&lt; 0.05) and E selectin (0.49 {\textpm} 0.1, P \&lt; 0.05) reduced the number of recruited T cells compared with IgG control (0.98 {\textpm} 0.1). Anti-E-selectin reduced the number of mature antigen-presenting cells trafficking to lymphoid tissue compared with control (6.96 {\textpm} 0.9 vs 12.67 {\textpm} 0.5, P \&lt; 0.05). Anti-E-selectin treatment delayed graft rejection and increased survival compared with control, although this difference did not reach statistical significance. CONCLUSIONS: In a model of corneal transplantation, P- and E-selectin mediate T cell recruitment to the graft, E-selectin mediates APC trafficking to lymphoid tissue, and blockade of E-selectin has a modest effect on improving long-term graft survival.},
	issn = {1534-6080},
	doi = {10.1097/TP.0000000000001107},
	author = {Dohlman, Thomas H and Di Zazzo, Antonio and Omoto, Masahiro and Hua, Jing and Ding, Julia and Hamrah, Pedram and Chauhan, Sunil K and Dana, Reza}
}
