@article {836946, title = {Tear Matrix Metalloproteinases and Myeloperoxidase Levels in Patients With Boston Keratoprosthesis Type I.}, journal = {Cornea}, volume = {35}, number = {7}, year = {2016}, month = {2016 Jul}, pages = {1008-14}, abstract = {PURPOSE: To investigate the tear levels of matrix metalloproteinases (MMPs), myeloperoxidase (MPO), and tissue inhibitor of metalloproteinase-1 in eyes after Boston keratoprosthesis type I (B-KPro) implantation and to correlate these markers with the established B-KPro prognostic categories. METHODS: Tear washes were collected from 40 patients (7 with autoimmune disease, 2 with chemical burn, and 31 with other noncicatrizing diagnoses). Tear levels of MMPs, MPO, and tissue inhibitor of metalloproteinase-1 were quantified using multianalyte bead-based enzyme-linked immunosorbent assays. The total MMP activity was determined using a fluorimetric assay. The analytes were compared to the underlying diagnosis and other clinical factors. RESULTS: The MMP-8, MMP-9, and MPO levels were markedly elevated in the eyes with B-KPro (80 {\textpm} 31, 291 {\textpm} 77, and 244 {\textpm} 33 pg/μg, respectively). Chemical burn was associated with significantly higher tear MMP-8 (474 {\textpm} 376 pg/μg) and MMP-9 levels (1300 {\textpm} 635 pg/μg) compared with noncicatrizing diseases (MMP-8: 41 {\textpm} 15 pg/μg, P = 0.02 and MMP-9: 196 {\textpm} 57 pg/μg, P = 0.02) and higher MMP-9 levels compared with autoimmune diseases (MMP-8: 96 {\textpm} 65 pg/μg, P = 0.21 and MMP-9: 306 {\textpm} 196 pg/μg, P = 0.04). Similar analyte levels were observed in the B-KPro eye and the contralateral non-B-KPro eye of patients with bilateral diseases. MMP-8, MMP-9, and total MMP activities correlated strongly with each other. CONCLUSIONS: In the eyes with B-KPro, tear MMP-8 and MMP-9 levels seem to be related to the underlying ocular surface pathology and not significantly influenced by the presence of the prosthesis.}, issn = {1536-4798}, doi = {10.1097/ICO.0000000000000893}, author = {Robert, Marie-Claude and Arafat, Samer N and Spurr-Michaud, Sandra and Chodosh, James and Dohlman, Claes H and Gipson, Ilene K} }