%0 Journal Article %J Methods Mol Biol %D 2013 %T Methods for culture of human corneal and conjunctival epithelia %A Spurr-Michaud, Sandra J %A Gipson, Ilene K %K Biomarkers %K Cell Culture Techniques %K Cell Differentiation %K Cell Line %K Cell Proliferation %K Conjunctiva %K Cornea %K Epithelial Cells %K Feeder Cells %K Humans %X The surface of the eye is exposed to the outside world and is, thus, subject to surface abrasion, infections, and drying, cicatrizing diseases. Availability of in vitro methods for culture of the human corneal and conjunctival epithelia, which cover the ocular surface, is therefore important in understanding the biology of these epithelia and their response to disease/infections, as well as for providing human-relevant models for preclinical testing of potential therapeutic agents. The ensuing chapter describes several methods for primary culture of both corneal and conjunctival epithelia and culture of immortalized cell lines, and methods employed to induce differentiation in the cultured epithelia. %B Methods Mol Biol %V 945 %P 31-43 %8 2013 %G eng %1 http://www.ncbi.nlm.nih.gov/pubmed/23097099?dopt=Abstract %R 10.1007/978-1-62703-125-7_3