%0 Journal Article %J Am J Pathol %D 2020 %T Resolvin E1 Reduces Leukotriene B4-Induced Intracellular Calcium Increase and Mucin Secretion in Rat Conjunctival Goblet Cells %A Yang, Menglu %A Bair, Jeffrey A %A Hodges, Robin R %A Serhan, Charles N %A Dartt, Darlene A. %K Animals %K Calcium %K Conjunctiva %K Eicosapentaenoic Acid %K Goblet Cells %K Leukotriene B4 %K Male %K Mucins %K Rats %K Rats, Sprague-Dawley %X Leukotriene B4 (LTB4) is a major proinflammatory mediator important in host defense, whereas resolvins (Rvs) are produced during the resolution phase of inflammation. The authors determined the actions of both RvE1 and RvD1 on LTB4-induced responses of goblet cells cultured from rat conjunctiva. The responses measured were an increase in the intracellular [Ca] ([Ca]) and high-molecular-weight glycoprotein secretion. Treatment with RvE1 or RvD1 for 30 minutes significantly blocked the LTB4-induced [Ca] increase. The actions of RvE1 on LTB4-induced [Ca] increase were reversed by siRNA for the RvE1 receptor, and the actions of RvD1 were reversed by an RvD1 receptor inhibitor. The RvE1 and RvD1 block of LTB4-stimulated increase in [Ca] was also reversed by an inhibitory peptide to β-adrenergic receptor kinase. LTB4 and block of the LTB4-stimulated increase in [Ca] by RvE1 and RvD1 were partially mediated by the depletion of intracellular Ca stores. RvE1, but not RvD1, counterregulated the LTB4-induced high-molecular-weight glycoprotein secretion. Thus, both RvE1 and RvD1 receptors directly inhibit LTB4 by phosphorylating the LTB4 receptor using β adrenergic receptor kinase. RvE1 receptor counterregulates the LTB4-induced increase in [Ca] and secretion, whereas RvD1 receptor only counterregulates LTB4-induced [Ca] increase. %B Am J Pathol %V 190 %P 1823-1832 %8 2020 09 %G eng %N 9 %1 http://www.ncbi.nlm.nih.gov/pubmed/32561135?dopt=Abstract %R 10.1016/j.ajpath.2020.06.001